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Press Release:
New high-throughput technology to map protein interactions
-Synthetic yeast cells enabled to visualize molecular networks of the cell-

  Cancer and many other human diseases are not the products of defects in a few genes or simple pathways. Rather, they involve a complex network of molecular interactions that are dynamically regulated. Thus, a technology that captures the regulated dynamics of a large-scale protein interaction network would be important to accelerate our understanding of complex disease mechanisms. Harnessing Synthetic Biology and computational approaches, Associate Professor Nozomu Yachie (Synthetic Biology) at the Research Center for Advanced Science and Technology, the University of Tokyo and researchers at University of Toronto have co-developed a high-throughput protein interaction technology BFG-Y2H. This new technology utilizing synthetic yeast cells as massive “bio-vials” achieved a higher throughput than ever before to identify protein interactions of the cell. Using BFG-Y2H, the researchers demonstrated that human protein interactions amongst ~2.5 million protein pairs can be screened by a single researcher in a few weeks. The new technology increases the efficiency of protein matrix screening, with quality that is on par with state-of-the-art protein interaction methods, and opens the door towards new biological researches underlying a big data of macromolecular webs.The results of this research were published on Molecular Systems Biology, Vol 12, Number 4.

Figure:BFG-Y2H technology enables high-throughput screening of molecular protein networks

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